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KMID : 0359319910310020155
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Korean Journal of Veterinary Research
1991 Volume.31 No. 2 p.155 ~ p.161
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Culture of glial cells isolated from the spinal cord of demyelinating mice infected with Theiler´s virus : An immunocytochemical study
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Abstract
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The mechanisms of demyelination in Theiler´s murine encephalomyelitis virus (TMEV)-induced chronic central nervous system(CNS) disease are still unclear and are probably multifactoral. This study was intended to culture spinal cord cells isolated from TMEV-induced demyelinating mice. By Percoll density centrifugation of enzymatically dissociated tissue, the cells were collected and then cultured on poly-L-lysine-coated plastic coverslips for 2 weeks.
Oligodendrocytes, astrocytes and macrophages were identified using cell-type specific markers. Viral antigens were not present in oligodendrocytes and in astrocytes by double immunofluorescence. Affected mouse oligodendrocytes had less capacities of sheet formation and galactocerebroside immunoreactivity than those of control cells. These findings support the hypothesis that immune mediated mechanisms play an important role in the process of demyelination in this animal model.
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KEYWORD
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